快速高效提取糞便DNA，可裂解任何糞便樣品。可直接用于PCR等其下游實(shí)驗(yàn)，配合FastPrep儀器使用無(wú)需研磨樣品。
產(chǎn)品名稱：糞便DNA試劑盒FastDNA Spin Kit For Feces(MP)
產(chǎn)品貨號(hào)：116570200
儲(chǔ)存溫度：15℃－30℃
The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.
Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
產(chǎn)品應(yīng)用，如下：
The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
產(chǎn)品屬性，如下：
|
Product Type |
Kits & Assays |
|
Components |
Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each |
|
Instrument Compatibility |
FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1 |
|
Sample Type |
Soil; Feces; Environmental Water; Wastewater; Sludge |
|
Sample Volume |
up to 500 mg per prep |
|
Tube Compatibility |
2 mL |
|
Matrix Type |
E |
|
Molecule Enriched |
DNA |
北京
